2018-02-10T15:57:16.000Z2025-05-05 11:33:43
locus
BSU07060
BSU_07060
geneLength
1836
1839
ec
outlinks
bsu
BSU07060
BSU_07060
Gene
Coordinates
772,142 → 773,980
772,142 773,980
The protein
Protein family
polysaccharide lyase 11 family (according to Swiss-Prot)
polysaccharide lyase 11 family (with [[protein|YesW]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|YesW]]
[[this]]
Biological materials
Mutant
MGNA-B261 (yesX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1260 NBRP B. subtilis, Japan]
BKE07060 (Δ[[gene|yesX]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATGGGAACGGGCTG, downstream forward: _UP4_TAGGAGGACTAAGGGATAAG
BKK07060 (Δ[[gene|yesX]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATGGGAACGGGCTG, downstream forward: _UP4_TAGGAGGACTAAGGGATAAG
MGNA-B261 (yesX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1260 NBRP B. subtilis, Japan]
BKE07060 ([[gene|yesX]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATGGGAACGGGCTG, downstream forward: _UP4_TAGGAGGACTAAGGGATAAG
BKK07060 ([[gene|yesX]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATGGGAACGGGCTG, downstream forward: _UP4_TAGGAGGACTAAGGGATAAG
The protein
Catalyzed reaction/ biological activity
Exotype eliminative cleavage of alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I oligosaccharides containing alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The products are the disaccharide 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose and the shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end (according to UniProt)